Yes, however, being written in Python, s3cmd requires Python 2.4+ for Windows to be installed and also it requires the Python library date-util.
Alternatively, you can try S3Express. S3Express is a "native" Windows command line program, that works out of the box and does not require any additional library or software to run. S3Express is a commercial program. It's very compact and has very small footprint: the entire program is less than 5MB. S3Express is perfect for uploading, querying, listing Amazon S3 objects via the command line on Windows.